A second search warrant was executed at the Toronto Humane Society on Tuesday by the Ministry of Natural Resources as the probe into the animal shelter deepens.

Investigators with the ministry are looking for information surrounding the release of two raccoons outside the proper jurisdiction.

It is alleged that the THS released the wild animals outside of the area where they were found. The law states that they must be released within a one-kilometre radius in order to limit the spread of rabies and other diseases, officials said.

The warrant comes nearly a week after officials from the Ontario Society for the Prevention of Cruelty to Animals executed their own search inside the River Street facility after the shelter's president and four senior staffers were charged with animal cruelty.

OSPCA investigator Kevin Strooband told reporters they are cooperating with ministry officials to make sure they get the information they are looking for.

He said all the wild animals that were in the shelter - eight in total - have been transferred out of the shelter and are now in the care of the Toronto Wildlife Centre where they are being treated by a veterinarian.

The ministry's warrant expires at 6 p.m. Tuesday, Strooband said. The OSPCA warrant is open ended with no set deadline.

A note on the THS website accused the OSPCA of operating illegally. The note said that the OSPCA warrant expires at the stroke of midnight Tuesday morning.

On Monday, an OSPCA lawyer went to court to clarify the conditions of the warrant and assured the public that investigators have the right to continue their search of the premises.

Strooband said he was at the premises last night at midnight but nobody from the THS showed up.

"We had no issues," he said.

The OSPCA has checked about 25 per cent of the 1,000-plus animals that are being sheltered at the THS. They are also searching computer records.

"We are still progressing today. We are working in different areas of the building," said Strooband.

The investigator said it may take up to three weeks for the OSPCA to complete their search but he said he hopes to reopen the shelter sooner for members of the public who want to adopt the animals.

Volunteers, vets and even some former THS staff members are helping look after the animals in the meantime.

The province has also stepped in this week, sending a letter to the THS board of directors, through the Office of the Public Guardian and Trustee. The letter warned the directors about using the charity's donations to pay for their legal fees. Doing so could contravene Ontario's Charities Accounting Act.